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Registros recuperados: 7
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Caracterização fenotípica pelo antibiograma de cepas de Escherichia coli isoladas de manipuladores, de leite cru e de queijo "Minas Frescal" em um laticínio de Goiás, Brasil Ciência Rural
Maria Raquel Hidalgo,Campos; Kipnis,André; André,Maria Cláudia Dantas Porfírio Borges; Vieira,Carla Atavila da Silva; Jayme,Liana Borges; Santos,Patrícia Pimentel; Serafini,Álvaro Bisol.
Este trabalho objetivou caracterizar fenotipicamente isolados de Escherichia coli a partir de amostras obtidas de mãos e narizes de manipuladores, de leite cru e de queijo Minas Frescal produzidos em um laticínio em Goiás, Brasil, visando a estabelecer uma relação de contaminação entre o manipulador e/ou a matéria-prima e o produto final. Foram coletadas 24 amostras de leite cru e de queijo Minas Frescal, 46 swabs de mãos e 46 de narizes dos manipuladores envolvidos na fabricação do queijo, no período entre março/2004 e fevereiro/2005. As 69 cepas isoladas foram submetidas ao teste de suscetibilidade a seis antimicrobianos (ampicilina, cefalotina, ciprofloxacina, gentamicina, sulfametoxazol trimetoprin e tetraciclina) e, a partir deste teste, foi possível...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coliformes; Manipulação; Leite cru; Queijo Minas Frescal.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400027
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Extensively drug-resistant tuberculosis: a case report and literature review BJID
Araújo-Filho,João Alves de; Vasconcelos-Jr,Arioldo Carvalho; Sousa,Eduardo Martins de; Silveira,Colombina da; Ribeiro,Elisangela; Kipnis,André; Junqueira-Kipnis,Ana Paula.
Extensively drug-resistant tuberculosis (XDR-TB) is an emerging health problem that threatens tuberculosis (TB) control worldwide, since suitable treatment for this disease has not yet been found. We report a case of secondary pulmonary XDR-TB in a 54-year-old, HIV-negative male from Goiânia, Brazil. The patient had long-standing pulmonary tuberculosis (nine years) with extensive bilateral lung damage and had been treated with multiple antituberculosis drugs (self-administered) before XDR-TB diagnosis. The strain of Mycobacterium tuberculosis was resistant to R- rifampicin, H-isoniazid, E-ethambutol, Eto-ethionamide, Ofx-ofloxacin, and Am-amikacin. This patient died with multiple organ failure due to sepsis secondary to bacterial pneumonia.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extensively drug-resistant tuberculosis; XDR-TB; Tuberculosis; Mycobacterium tuberculosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500019
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Health care workers humoral immune response against GLcB, MPT51 and HSPX from Mycobacterium tuberculosis BJID
Reis,Michelle Cristina Guerreiro dos; Rabahi,Marcelo Fouad; Kipnis,André; Junqueira-Kipnis,Ana Paula.
Tuberculosis (TB) is one of the oldest human infectious diseases and one third of the world's population is latently infected. Brazil is an endemic area for TB. One of the most important challenges in TB control is the identification of latently infected individuals. Health Care Workers (HCW) are at high risk of being infected with Mycobacterium tuberculosis and even to become TB latently infected. The aim of this study was to increase knowledge about humoral immune response in TB latently infected individuals. HCW were classified according to their tuberculin skin test (TST), as positive or negative. The antibody response to GLcB, MPT51 and HSPX from Mycobacterium tuberculosis was evaluated. TST negative HCW constituted the majority of those who showed a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Latent tuberculosis; Infection; Antibodies.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600006
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Methicillin-resistant Staphylococcus aureus nasal carriage in neonates and children attending a pediatric outpatient clinics in Brazil BJID
Vieira,Maria Aparecida; Minamisava,Ruth; Pessoa-Júnior,Vicente; Lamaro-Cardoso,Juliana; Ternes,Yves Mauro; Andre,Maria Cláudia Porfirio; Sgambatti,Sabrina; Kipnis,André; Andrade,Ana Lúcia.
Background:In Latin America, few studies have been carried out on methicillin-resistant Staphylococcus aureus carriage in the pediatric population. We conducted a survey of nasal S. aureus carriage in neonates and in children attending the pediatric outpatient clinics in a large Brazilian city with high antimicrobial consumption.Methods:Pernasal swabs of neonates were collected upon admission and at discharge in four neonatal intensive care units and of children less than five years of age during outpatient visits. Methicillin-resistant S. aureus isolates were characterized for antibiotic susceptibility, mec gene presence, pulsed-field gel electrophoresis, spatype, SCCmec-type, multilocus sequence type, and presence of Panton-Valentine leukocidin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus carriage; Methicillin-resistant Staphylococcus; Aureus; Neonates; Children.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100042
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Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital BJM
Costa,Dayane de Melo; Kipnis,André; Leão-Vasconcelos,Lara Stefânia Netto de Oliveira; Rocha-Vilefort,Larissa Oliveira; Telles,Sheila Araújo; André,Maria Cláudia Dantas Porfírio Borges; Tipple,Anaclara Ferreira Veiga; Lima,Ana Beatriz Mori; Ribeiro,Nádia Ferreira Gonçalves; Pereira,Mayara Regina; Prado-Palos,Marinésia Aparecida.
The aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and saliva sampling were performed with 149 health care workers. Antimicrobial resistance was determined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were performed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were determined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Health personnel; Occupational health; Staphylococcus; Methicillin resistance; Oncology service; Hospital.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300006
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Multidrug-resistant tuberculosis: case reports study in a central state of Brazil BJID
Araújo-Filho,João Alves de; Vasconcelos-Jr,Arioldo Carvalho; Sousa,Eduardo Martins de; Silveira,Colombina da; Sousa,Patrícia Tavares Pereira de; Severo,Klaus Andrade; Vieira,Ludmila Ferreira; Kipnis,André; Junqueira-Kipnis,Ana Paula.
Multidrug-resistant tuberculosis (MDR-TB) is an emerging and worrisome health problem that threatens tuberculosis (TB) control worldwide. The clinical management of MDR-TB is a complex issue associated with the use of multiple drugs for a long period, usually accompanied by side effects and high costs. The objective of this work was to relate cases of MDR-TB occurring in Goiás, a central state of Brazil. We related five cases of MDR-TB, three women and two men. All were pulmonary cases. Three were in their second treatment and two in their first treatment. Surgical pulmonary resection was performed in one case. One death occurred. Lack of adherence, gastric intolerance to anti-TB drugs and poor clinical management were the main aspects related to the...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Tuberculosis; Multidrug-resistant tuberculosis; MDR-TB; Mycobacterium tuberculosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100020
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New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli BJM
Xavier,Mauro Aparecido Souza; Kipnis,André; Torres,Fernando Araripe Gonçalves; Astofi-Filho,Spartaco.
We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heterologous expression; Escherichia coli; Molecular cloning; Induced expression.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400007
Registros recuperados: 7
Primeira ... 1 ... Última
 

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